Synthesis and activity of new triphenylphosphonium derivatives of betulin and betulinic acid against Schistosoma mansoni in vitro and in vivo

We studied the antischistosomal activity of betulin, betulinic acid and its 9 triphenylphosphonium derivatives characterized by a covalently linkage of the hydrophobic fragment of triterpenoid at C(2)- or C(30)-position with the triphenylphosphonium moiety via a hydrocarbon bridge. The triphenylphosphonium salts showed in vitro antischistosomal activity against newly transformed schistosomula (NTS) and adult worms of Schistosoma mansoni at low micromolar concentrations. In contrast betulin and betulinic acid were inactive against NTS and adult S. mansoni. Of the 9 triphenylphosphonium derivatives tested, the allyl salts 10 (IC₅₀ of 0.76 μg/mL) and 11 (IC₅₀ of 0.64 μg/mL) demonstrated the highest antischistosomal activity against adult S. mansoni. Low worm burden reductions of 22% were observed in vivo for these two compounds. In conclusion, triphenylphosphonium derivatives were obtained from available natural betulin by simple transformations, rendering it practical and useful for large scale application. However, further structural modifications are necessary to translate the promising antischistosomal in vitro activities into in vivo.     

A global synthesis of the effects of diversified farming systems on arthropod diversity within fields and across agricultural landscapes

Agricultural intensification is a leading cause of global biodiversity loss, which can reduce the provisioning of ecosystem services in managed ecosystems. Organic farming and plant diversification are farm management schemes that may mitigate potential ecological harm by increasing species richness and boosting related ecosystem services to agroecosystems. What remains unclear is the extent to which farm management schemes affect biodiversity components other than species richness, and whether impacts differ across spatial scales and landscape contexts. Using a global metadataset, we quantified the effects of organic farming and plant diversification on abundance, local diversity (communities within fields), and regional diversity (communities across fields) of arthropod pollinators, predators, herbivores, and detritivores. Both organic farming and higher in‐field plant diversity enhanced arthropod abundance, particularly for rare taxa. This resulted in increased richness but decreased evenness. While these responses were stronger at local relative to regional scales, richness and abundance increased at both scales, and richness on farms embedded in complex relative to simple landscapes. Overall, both organic farming and in‐field plant diversification exerted the strongest effects on pollinators and predators, suggesting these management schemes can facilitate ecosystem service providers without augmenting herbivore (pest) populations. Our results suggest that organic farming and plant diversification promote diverse arthropod metacommunities that may provide temporal and spatial stability of ecosystem service provisioning. Conserving diverse plant and arthropod communities in farming systems therefore requires sustainable practices that operate both within fields and across landscapes.     

Management of ecosystem services in mountain forests: Review of indicators and value functions for model based multi-criteria decision analysis

The main ecosystem services (ES) central European mountain forests provide are: protection against gravitational hazards, timber production, recreation, biodiversity conservation and carbon storage, which are all in high demand. These demands make managing mountain forests a challenging task, involving manifold synergies and conflicts between the different ES. There is therefore an urgent need for appropriate concepts and tools for support decisions in forest management and planning (FMP) to take into consideration all ES and to manage the wide variety of information types, parameters and uncertainties involved in assessing the sustainability of ES. Multi-criteria decision analysis (MCDA) provides a suitable set of methods for sustainability evaluations. In this study sustainability means the persistent fulfilment of the required ES. To address all the phases of the FMP process, MCDA and forest models should be applied together, with indicators providing the main interfaces to combine them. This paper aims to: i) review assessment approaches in order to select appropriate and widely accepted indicators for measuring and assessing the effects of different silvicultural management alternatives on forest ES, and ii) present additional standardisation approaches (value functions) for each indicator. Standardisations are necessary to make the different ES comparable and to study synergies and trade-offs between different management objectives in MCDA. The main ES in central European mountain regions are considered, with a clear focus on those indicators that are directly derivable from forest model outputs and that can refer to sustainable forest management practices. The scales considered are that of the single forest stand and of the larger forest management unit. A holistic indicator-based analysis framework for FMP in mountain forests can be built using the indicators and value functions described. The influence of different management alternatives on ES can then be evaluated, taking into consideration the instruments and information on forest management (forest models, inventory) available. All indicators are selected according to existing and approved approaches that only require data that is normally available in operational forest management. The framework can thus be an important element in developing a decision support system for FMP in mountain forests.     

The synthesis and spectroscopic analysis of the neurotoxic prion peptide 106-126: Comparative use of manual Boc and Fmoc chemistry

A peptide corresponding to residues 106-126 of the human prion protein (PrP) possesses the neurotoxic and amyloidogenic properties of the infectious form of the parental protein. This peptide is now identified as a 'difficult sequence' and synthesis using conventional manual Fmoc chemistry was unsuccessful with acylation terminating at a central core of hydrophobic amino acids. The use of tetramethylfluoroformamidinium hexafluorophosphate and 1-methyl-2- pyrrolidone as anti-aggregatory agents in the coupling steps improved the synthesis but still resulted in an incomplete peptide. The incorporation of N-(2-hydroxy-4-methoxybenzyl) protection at glycine residues 119 and 124 enabled synthesis of the full length peptide in low yield. Synthesis using Boc chemistry with in situ neutralisation gave the full length peptide in high yield.     

Development of mating disruption for control of pine sawfly populations

Mating disruption of the pine sawflyNeodiprion sertifer (Geoffroy) (Hymenoptera: Diprionidae) was strongly indicated by reduced male trap catches in pine plantations permeated with the sex phermone, (2S, 3S, 7S)-diprionly acetate. The trap catch reduction was 95 to near 100% when dispensers every 10 m were used, giving a total release of about 3 mg per hectare and day. Two mg of pheromone per cotton roll dispenser maintained low catches for the whole season (about 2 months) without any renewal of disruption dispensers. Anerythro-mixture was as effective as the pure pheromone isomer. The effects of the experiments on population density and sex ratio were not possible to investigate, due to a general collapse of the population, also outside experimental plots, the year after the experiments.     

Interpreting coral reef monitoring data: A guide for improved management decisions

Coral reef monitoring programmes exist in all regions of the world, recording reef attributes such as coral cover, fish biomass and macroalgal cover. Given the cost of such monitoring programs, and the degraded state of many of the world’s reefs, understanding how reef monitoring data can be used to shape management decisions for coral reefs is a high priority. However, there is no general guide to understanding the ecological implications of the data in a format that can trigger a management response. We attempt to provide such a guide for interpreting the temporal trends in 41 coral reef monitoring attributes, recorded by seven of the largest reef monitoring programmes. We show that only a small subset of these attributes is required to identify the stressors that have impacted a reef (i.e. provide a diagnosis), as well as to estimate the likely recovery potential (prognosis). Two of the most useful indicators, turf algal canopy height and coral colony growth rate are not commonly measured, and we strongly recommend their inclusion in reef monitoring. The diagnosis and prognosis system that we have developed may help guide management actions and provides a foundation for further development as biological and ecological insights continue to grow.     

Advanced water splitting for green hydrogen gas production through complete oxidation of starch by in vitro metabolic engineering

Starch is a natural energy storage compound and is hypothesized to be a high-energy density chemical compound or solar fuel. In contrast to industrial hydrolysis of starch to glucose, an alternative ATP-free phosphorylation of starch was designed to generate cost-effective glucose 6-phosphate by using five thermophilic enzymes (i.e., isoamylase, alpha-glucan phosphorylase, 4-α-glucanotransferase, phosphoglucomutase, and polyphosphate glucokinase). This enzymatic phosphorolysis is energetically advantageous because the energy of α-1,4-glycosidic bonds among anhydroglucose units is conserved in the form of phosphorylated glucose. Furthermore, we demonstrated an in vitro 17-thermophilic enzyme pathway that can convert all glucose units of starch, regardless of branched and linear contents, with water to hydrogen at a theoretic yield (i.e., 12 H₂ per glucose), three times of the theoretical yield from dark microbial fermentation. The use of a biomimetic electron transport chain enabled to achieve a maximum volumetric productivity of 90.2 mmol of H₂/L/h at 20 g/L starch. The complete oxidation of starch to hydrogen by this in vitro synthetic (enzymatic) biosystem suggests that starch as a natural solar fuel becomes a high-density hydrogen storage compound with a gravimetric density of more than 14% H₂-based mass and an electricity density of more than 3000 W h/kg of starch.     

Geographical range size of tropical plants influences their response to anthropogenic activities

Biodiversity patterns may be influenced by the species geographical range sizes, but this is rarely shown. We used a highly replicated and large-scale study in coastal Ecuador to determine for the first time the importance of latitudinal range size of plant species in their response to land-use activities. We examined herbaceous plant communities of five land-use types with decreasing anthropogenic disturbance (from the most intervened rice and pasture to the less intervened managed agroforest, abandoned agroforest, and forest) in a low and a high impact human-dominated landscape. All species were classified in four latitudinal range size quartiles, from the 25% species with the narrowest to the 25% with the widest range size. We found notable differences between patterns of total species richness and those of individual range size quartiles. Whereas total species richness was higher in more intervened land-use types, percentages of narrow-ranged species were significantly higher in less intervened land-use types. In contrast, percentages of wide-ranging species were higher in more intervened land-use types. Hence, responses of plant species to human activities were influenced by traits that determine their range sizes. An analysis of floristic similarity between land-use types revealed that narrow-ranged species were mainly preserved in forest fragments, but the other land-use types supported many unique narrow-ranged species and therefore made an important contribution to their preservation at the landscape level. Conservation efforts should combine protection of natural habitats with strategies to maintain a diversity of low-intensity land-use types, looking for win-win solutions or trade-offs between biodiversity conservation and human welfare in human-dominated landscapes.     

A linkage group on pig chromosome 4 comprising the loci for blood group L, GBA, ATP1B1 and three microsatellites

Restriction fragment length polymorphisms (RFLPs) were described for the porcine loci for β-glucosidase (GBA) and the β-polypeptide 1 of the Na⁺, K⁺-transporting ATPase (ATP1B1). Linkage analyses using a three-generation pedigree provided evidence for the assignment of ATP1B1, GBA and two microsatellite loci (S0001 and S0067) to a previously described linkage group comprising the loci for blood group L (EAL) and an anonymous microsatellite (S0097). The linear order of the six markers was determined with confidence by multipoint analyses and the length of the linkage group was estimated at 88 CM. This linkage group was assigned to pig chromosome 4 on the basis of a previous physical localization of the ATP1B1 gene. In situ hybridization data for S0001 presented in this study were consistent with a localization on chromosome 4 and suggested a regional localization to 4pl2-pl3. The present study reveals conflicting data concerning the genetic localization of the K88 loci controlling the expression of the receptors for the E. coli pilus antigens. One group has reported data suggesting a loose linkage between K88 and EAL, now mapped to chromosome 4, whereas two other groups have found linkage between K88 and the transferrin locus (TF), mapped to chromosome 13 by in situ hybridization.